Associations Between Post-Wingate Blood Gas and Acid–Base Changes and Anaerobic Performance in Elite Male Marathon Runners
Elit Erkek Maraton Koşucularında Wingate Anaerobik Güç Testi Sonrası Kan Gazı ve Asit–Baz Yanıtlarının Anaerobik Performans ile İlişkisi
Tolga ALTUĞ1
*Correspondence: Tolga ALTUĞ E-mail: taltug@agri.edu.tr
1Ağrı İbrahim Çeçen University, Faculty of Sport Sciences, Ağrı, Türkiye E-mail: taltug@agri.edu.tr ORCID: https://orcid.org/0000-0001-6318-0107
Doi: https://doi.org/10.5281/zenodo.18909877
https://www.ijoss.org/Archive/v3-i1/ijoss-Volume3-issue1-20.pdf
Received / Gönderim: 25.12.2025 Accepted / Kabul: 25.02.2026 Published / Yayın: 28.02.2026
Abstract
Purpose: This study investigated post-Wingate changes in blood gas and acid–base parameters in elite male marathon runners competing at the Olympic level. Associations between these responses and anaerobic performance indicators were also explored. Methods: Elite male marathon runners (n = 28) voluntarily participated in the study. All participants completed a standard Wingate Anaerobic Power Test during a single testing session. Mean power output and fatigue index were recorded as indicators of anaerobic performance. Blood gas and acid–base parameters were measured immediately before the test and at three minutes post-exercise. Pre–post comparisons were analysed using paired-samples t-tests, and correlations between performance indicators and physiological variables were examined using Pearson correlation analysis. Results: Following the Wingate test, statistically significant changes were observed in pH, pO₂, pCO₂, and HCO₃⁻ values (p < 0.001), whereas the increase in lactate levels was not statistically significant (p > 0.05). A significant positive correlation was identified between mean power and ΔpO₂ (p < 0.05), while no other significant associations were observed. Conclusion: Overall, the findings indicate that although the Wingate test induces measurable systemic acid–base alterations in elite marathon runners, these physiological responses show only limited associations with anaerobic performance indicators.
Keywords Acid–base balance, anaerobic performance, blood gas analysis, marathon runners, Wingate test.
ÖZ
Amaç: Bu çalışma, Olimpik düzeyde yarışan elit erkek maraton koşucularında Wingate testi sonrası kan gazı ve asit–baz parametrelerindeki değişimleri incelemeyi amaçlamıştır. Ayrıca bu fizyolojik yanıtların anaerobik performans göstergeleri ile olan ilişkileri de araştırılmıştır. Yöntem: Çalışmaya elit erkek maraton koşucuları (n = 28) gönüllü olarak katılmıştır. Tüm katılımcılar tek bir test oturumunda standart Wingate Anaerobik Güç Testini tamamlamıştır. Anaerobik performans göstergeleri olarak ortalama güç çıktısı ve yorgunluk indeksi kaydedilmiştir. Kan gazı ve asit–baz parametreleri testten hemen önce ve egzersizden üç dakika sonra ölçülmüştür. Ön test–son test karşılaştırmaları eşleştirilmiş örneklemler t-testi ile analiz edilmiş, performans göstergeleri ile fizyolojik değişkenler arasındaki ilişkiler Pearson korelasyon analizi ile incelenmiştir. Bulgular: Wingate testi sonrasında pH, pO₂, pCO₂ ve HCO₃⁻ değerlerinde istatistiksel olarak anlamlı değişimler gözlenmiştir (p < 0.001). Buna karşın laktat düzeylerindeki artış istatistiksel olarak anlamlı bulunmamıştır (p > 0.05). Ortalama güç ile ΔpO₂ arasında anlamlı pozitif bir korelasyon tespit edilmiş (p < 0.05), diğer değişkenler arasında ise anlamlı bir ilişki bulunmamıştır. Sonuç: Genel olarak bulgular, Wingate testinin elit maraton koşucularında ölçülebilir sistemik asit–baz değişikliklerine yol açtığını; ancak bu fizyolojik yanıtların anaerobik performans göstergeleri ile yalnızca sınırlı düzeyde ilişkili olduğunu göstermektedir.
Anahtar Kelimeler Asit–baz dengesi, anaerobik performans, kan gazı analizi, maraton koşucuları, Wingate.
- Acar, N., Umutlu, G., Ersöz, Y., Taşman, G., Güven, E., Ulutaş, D., Kamiş, O., Erdoğan, M., & Aslan, Y. (2025). Continuous vertical jump test is a reliable alternative to Wingate anaerobic test and isokinetic fatigue tests in evaluation of muscular fatigue resistance in endurance runners. BMC Sports Science, Medicine and Rehabilitation, 17. https://doi.org/10.1186/s13102-025-01143-0
- Allen, D. G. (2020). Human muscle performance. The Journal of Physiology. https://doi.org/10.1113/JP279364
- Allen, D. G., Lamb, G. D., & Westerblad, H. (2008). Skeletal muscle fatigue: Cellular mechanisms. Physiological Reviews, 88(1), 287–332. https://doi.org/10.1152/physrev.00015.2007
- Álvarez-Herms, J. (2024). Summatory effects of anaerobic exercise and a “Westernized athletic diet” on gut dysbiosis and chronic low-grade metabolic acidosis. Microorganisms, 12(6), 1138. https://doi.org/10.3390/microorganisms12061138
- Amann, M., & Dempsey, J. A. (2008). Locomotor muscle fatigue modifies central motor drive in healthy humans and imposes a limitation to exercise performance. The Journal of Physiology, 586(1), 161–173. https://doi.org/10.1113/jphysiol.2007.141838
- Amann, M., Wan, H.-Y., Thurston, T. S., Georgescu, V. P., & Weavil, J. C. (2020). On the influence of group III/IV muscle afferent feedback on endurance exercise performance. Exercise and Sport Sciences Reviews, 48(4), 209–216. https://doi.org/10.1249/JES.0000000000000233
- Bar-Or, O. (1987). The Wingate anaerobic test: An update on methodology, reliability and validity. Sports Medicine, 4(6), 381–394. https://doi.org/10.2165/00007256-198704060-00001
- Beneke, R., Pollmann, C., Bleif, I., Leithäuser, R. M., & Hütler, M. (2002). How anaerobic is the Wingate anaerobic test for humans? European Journal of Applied Physiology, 87(4–5), 388–392. https://doi.org/10.1007/s00421-002-0622-4
- Cairns, S. P., & Lindinger, M. I. (2025). Lactic acidosis: Implications for human exercise performance. European Journal of Applied Physiology, 125(7), 1761–1795. https://doi.org/10.1007/s00421-025-05750-0
- Calbet, J. A. L., De Paz, J. A., Garatachea, N., De Vaca, S. C., & Chavarren, J. (2003). Anaerobic energy provision does not limit Wingate exercise performance in endurance-trained cyclists. Journal of Applied Physiology, 94(2), 668–676. https://doi.org/10.1152/japplphysiol.00128.2002
- Castañeda-Babarro, A. (2021). The Wingate anaerobic test: A narrative review of the protocol variables that affect the results obtained. Applied Sciences, 11(16), 7417. https://doi.org/10.3390/app11167417
- Cohen, J. (1988). Statistical power analysis for the behavioral sciences (2nd ed.). Hillsdale, NJ: Lawrence Erlbaum Associates. https://doi.org/10.4324/9780203771587
- Dobashi, K., Fujii, N., Ichinose, M., & Nishiyasu, T. (2021). Voluntary hypocapnic hyperventilation lasting 5 min and 20 min similarly reduce aerobic metabolism without affecting power outputs during Wingate anaerobic test. European Journal of Sport Science, 21(8), 1148–1155. https://doi.org/10.1080/17461391.2020.1812728
- Driss, T., & Vandewalle, H. (2013). The measurement of maximal (anaerobic) power output on a cycle ergometer: A critical review. BioMed Research International, 2013, 589361. https://doi.org/10.1155/2013/589361
- Fujii, N., Tsuchiya, S.-I., Tsuji, B., Watanabe, K., Sasaki, Y., & Nishiyasu, T. (2015). Effect of voluntary hypocapnic hyperventilation on the metabolic response during Wingate anaerobic test. European Journal of Applied Physiology, 115, 1967–1974. https://doi.org/10.1007/s00421-015-3179-8
- Hureau, T. J., Broxterman, R. M., Weavil, J. C., Lewis, M. T., Layec, G., & Amann, M. (2022). On the role of skeletal muscle acidosis and inorganic phosphates as determinants of central and peripheral fatigue: A 31P-MRS study. The Journal of Physiology, 600(13), 3069–3081. https://doi.org/10.1113/JP283036
- Kisaka, T., Cox, T. A., Dumitrescu, D., & Wasserman, K. (2015). CO₂ pulse and acid–base status during increasing work rate exercise in health and disease. Respiratory Physiology & Neurobiology, 218, 46–56. https://doi.org/10.1016/j.resp.2015.07.005
- Medbø, J. I., Noddeland, H., & Hanem, S. (2012). Acid–base status of arterial and femoral venous blood during and after intense cycle exercise. Acta Physiologica Hungarica, 99(1), 66–94. https://doi.org/10.12697/akut.2009.14.06
- Mündel, T. (2018). Sodium bicarbonate ingestion improves repeated high-intensity cycling performance in the heat. Temperature, 5(4), 343–347. https://doi.org/10.1080/23328940.2018.1436393
- Nikolaidis, P. T., Rosemann, T., & Knechtle, B. (2018). Force–velocity characteristics, muscle strength, and flexibility in female recreational marathon runners. Frontiers in Physiology, 9. https://doi.org/10.3389/fphys.2018.01563
- Robergs, R. A., Ghiasvand, F., & Parker, D. (2004). Biochemistry of exercise-induced metabolic acidosis. American Journal of Physiology–Regulatory, Integrative and Comparative Physiology, 287(3), R502–R516. https://doi.org/10.1152/ajpregu.00114.2004
- Sahlin, K. (2014). Muscle energetics during explosive activities and potential effects of nutrition and training. Sports Medicine, 44(Suppl 2), S167–S173. https://doi.org/10.1007/s40279-014-0256-9
- Seiler, S., & Tønnessen, E. (2009). Intervals, thresholds, and long slow distance: The role of intensity and duration in endurance training. Sportscience, 13, 32–53.
- Shaw, I., & Gregory, K. (2022). Acid–base balance: A review of normal physiology. BJA Education, 22(10), 396–401. https://doi.org/10.1016/j.bjae.2022.06.003
- Souissi, N., Driss, T., Chamari, K., Vandewalle, H., Davenne, D., Gam, A., Fillard, J. R., & Jousselin, E. (2010). Diurnal variation in Wingate test performances: Influence of active warm-up. Chronobiology International, 27(3), 640–652. https://doi.org/10.3109/07420528.2010.483157
- Sousa, C. V., Sales, M. M., Nikolaidis, P. T., Rosemann, T., & Knechtle, B. (2018). How much further for the sub-2-hour marathon? Open Access Journal of Sports Medicine, 9, 139–145. https://doi.org/10.2147/OAJSM.S169758
- Stefanova, D., & Petrova, B. (2017). Wingate anaerobic test: Additional physiological interpretation. Journal of Applied Sports Sciences, 1(2), 50–62. https://doi.org/10.37393/JASS.2017.02.6
- Sun, X., Hansen, J. E., Stringer, W. W., Ting, H., & Wasserman, K. (2001). Carbon dioxide pressure–concentration relationship in arterial and mixed venous blood during exercise. Journal of Applied Physiology, 90(5), 1798–1810. https://doi.org/10.1152/jappl.2001.90.5.1798
- Yel, K., & Güzel, S., Kurcan, K., Aydemir, U. (2023). Spor Performansı v Denge. Spor Araştırmalarında Farklı Perspektifler 2, Editör: Prof.Dr. Erdal Zorba, Doç.Dr. Mevlüt Gönen, Dr. Zekai ÇAKIR, Aralık. Bölüm-8, ISBN: 978-625-6643-01-7, Syf:120-137. Duvar Yayınları. İzmir.
